Developed by the co-inventors of the early Lipofectamine® products, DNA-In® Neuro Transfection reagent was formulated from a novel chemistry for maximum transfection efficiency in neurons. In side-by-side assays with top competitor reagents significantly higher transfection efficiencies are consistently observed when DNA-In® Neuro is used to transfect primary cortical, hippocampal or forebrain neurons. Moreover, with low cytotoxic effect DNA-In® Neuro supports neuronal survival and neurite extension post-transfection.
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Figure 1. High GFP Expression in Primary Neurons – Primary rat cortical neurons were transfected with DNA-In® Neuro Transfection Reagent and incubated overnight in complete culture media. The above images were taken 48-hours post-transfection and show uniform, high GFP expression in healthy cells.
Higher Transfection Efficiency - Two-fold or greater improvement in efficiency over competing transfection reagents
Exceptionally Low Toxicity - Maximum post-transfection neuron viability critical for performing assays on healthy, uncompromisedtransfected neurons.
Highly Robust Performance – Produces consistent and reproducible results.
Quick and Easy-to-Use – An easy to follow, single-tube reagent protocol for great results on the very first try.
DNA-In® Neuro Transfection Reagent is a new transfection reagent that consistently produces high transfection efficiencies in neurons, typically achieving a 2-fold or better improvement in efficiency over the competing reagents currently available, including Lipofectamine® 2000 and NeuroFECT™. Moreover, DNA-In® Neuro enables neurons to be efficiently transfected with minimal toxicity to support healthy post-transfected neurons, critical for performing assays on uncompromised transfected cells.
Figure 2. (Top,Bottom). DNA-In® Neuro Outperforms Leading Competitor Reagent– DNA-In® Neuro was used to transfect plasmid DNA encoding GFP into 6-day cultured E18 Primary Rat Cortical Neurons. Transfections were performed in 24-well plates using 1.0-3.0µl of DNA-In® Neuro Reagent. The above data show DNA-In® Neuro significantly outperforms Lipofectamine® 2000 with near 3-fold improvement in transfection efficiency after 24 hours (Bottom Graph). Duplicate wells were assayed 24-hrs and 48-hrs after transfection.
Figure 2. Western blot analysis of the transfected neurons using DNA-IN Neuro - Cortical neurons 5 days in culture were transfected with (1) p35and (2) p25 (pcDNA3.1 (-) Myc His A) plasmids in 6 well plates 3µg/well and using 12µL of DNA- In® Neuro neuron transfection reagent. After 48 hr, western blot analysis was performed using 30µg of cell lysates for each plasmid to show expression. Data courtesy of Dr. NiranjanaAmin, NIH, NINDS.
Our full product line of DNA-In® Cell-Specific Transfection Reagents is optimized for exceptionally high efficiency and cell viability performance in specific cell lines. The dosage, procedure and formulation of each of these reagents has been extensively tested and validated by our MTI-GlobalStem developers and other customers.
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