DYKDDDDK Fab-Trap™ Agarose是高效免疫沉淀(IP)Flag®标记蛋白质的高级亲和树脂。DYKDDDDK Fab-Trap™ Agarose由一个Fab片段组成,该片段与琼脂糖珠共价结合。
应用
Immunoprecipitation (IP) / Co-IP 免疫沉淀(IP)/共沉淀
Immunoprecipitation (IP) / Co-IP质谱(MS)
Protein purification 蛋白质纯化
ChIP/ RIP analysis ChIP/RIP分析
On-bead enzyme assays 微珠酶分析
实验结果
DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.
订货信息
DYKDDDDK Immunoprecipitation Starter Pack
DYKDDDDK Fab-Trap™ Agarose (20 reactions, product code ffa-20) for immunoprecipitation, 3xDYKDDDDK-peptide (1mg, fp-1) for elution and DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20UL) for detection in Western blots.
Starter Pack (ffap) 包括以下组分:
· DYKDDDDK Fab-Trap™ Agarose (20 reactions, ffa-20),
· 3xDYKDDDDK-peptide (1mg, fp-1) and
· DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20).
默瑞生物
默瑞(上海)生物科技有限公司,作为专业的生命科学产品推广与服务商,以“创新,专业,共赢”的理念,不断强化服务体系,提升客户体验,结合国内外优质产品提供高标准完整解决方案,帮助科研客户和生物技术企业高效创新,加速转化,创造更多优质产品,提升社会福祉,实现多方共赢。
默瑞生物创立以来,已经陆续导入TwistDx,enzymatics,KAPA BIOSYSTEMS,BioDynami,Jackson,Echelon,ChromoTek,Nanocs,Biotechrabbit,Lee Biosolutions, magtivio,美的医疗冰箱,FOUR E'S科学仪器等优质国际品牌,逐步培育成为专业用户的首选。
代理试剂产品有:TwistDx恒温扩增试剂盒,Echelon脂类研究试剂,KAPA BIOSYSTEMS二代测序试剂, Enzymatics二代测序酶,Jackson ImmunoResearch抗体,ChromoTek荧光蛋白及抗体,Horizon Discovery测序标准品,Biomatik公司Elisa试剂盒,Nanocs PEG修饰剂,Spectrumlabs透析袋,Polysciences微球,JenaBioscience分子试剂,Lifespan Technologies硫酸葡聚糖ELISA试剂盒及抗体,Lee Biosolutions抗体,Emfret心血管研究抗体,Magtivio B.V.DNA提取磁珠,Biontex Laboratories转染试剂,Canvax™细胞凋亡检测试剂盒,Matriks Biotek®生物药物检测试剂盒,Immunostep流式细胞术抗体,Fuller Laboratories感染性疾病检测试剂盒,SICGEN抗体,biotechrabbit酶及抗体等。