DNA-In® Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. These cell-specific reagents offer maximum DNA delivery in targeted common cell lines.
DNA-In® HepG2 Transfection Reagent provides maximum transfection efficiency, gene expression levels and cell viability in HepG2 cells, a human epithelial, hepatocellular carcinoma cell line, commonly used as a model to study of human liver diseases, including investigation of liver metabolism and toxicity.
Features
Fast, simple, easy-to-follow protocol
One-step process: simply add DNA-In® HepG2 Transfection Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells
Minimal toxicity - no need to replace medium after transfection
DNA-In® HepG2 Transfection Reagent Quick-Start Protocol
<img src="https://www.mti-globalstem.com/image/catalog/Transfection-MTI/DNAIn-Transfection-Protocol_Animated.gif" alt="DNA-In HepG2 transfection protocol>
During its development and validation process, DNA-In® HepG2 Transfection Reagent underwent extensive testing in HepG2 cells. In head-to-head comparison studies, DNA-In® HepG2 Reagent consistently outperformed Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagents to produce significantly higher transfection efficiency and cell viability.
The following charts show side-by-side comparisons of DNA-In® HepG2, Lipofectamine® 2000 (LF2K) and Lipofectamine® 3000 (LFK) in HepG2 cells:
DNA-In® HepG2 Transfection Reagent provides Maximum Transfection Efficiency in HepG2 cells
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Above, HepG2 cells were plated at 70% - 80% confluency. They were subsequently transfected with the EF-1 alpha promoter-GFP 24 hours after plating. Significantly higher efficiency was consistently observed in HepG2 cells transfected with DNA-In® HepG2 Reagent (left image) vs LF3K (right image) transfected cells.
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