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DNA-In® CHO Transfection Reagent

DNA-In® Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. These cel

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DNA-In® CHO Transfection Reagent

Formulated Specifically for CHO-K1 Cells 

DNA-In® Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. These cell-specific reagents offer maximum DNA delivery in targeted common cell lines. 

The cell-specific DNA-In® CHO Transfection Reagent provides maximum high transfection, expression levels and cell viability in adherent CHO-K1 cells. 


Features


  • Formulated specifically for maximum high transfection, expression levels and cell viability in adherent CHO-K1 cells. 

  • One-step process: simply add DNA-In® CHO Transfection Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells

  • Minimal toxicity - no need to replace medium after transfection


Maximum Transfection Efficiency in CHO-K1 Cells

Superior Transfection Efficiency and Maximum Cell Viability - During its development and validation process, DNA-In® CHO Transfection Reagent underwent extensive testing in many primary and cultured cells.  

dna-in cho transfectionlipofectamine 3000 transfection of cho cells

Above, adherent CHO-K1 cells were plated at ~70% confluency and transfected with the EF-1 alpha promoter-GFP after 24 hours in culture. DNA-In™ CHO transfected cells (left image) show significantly better transfection efficiency and cell viability as compare to LF3K transfected cells (right Image). 

comparison of dna-in cho transfection vs lipofectamine

Above data shows a side-by-side comparisons of DNA-In® CHO Transfection Reagent, Lipofectamine® 2000 (LF2K) and Lipofectamine® 3000 (LFK) in CHO-K1 cells In a head-to-head comparison study, DNA-In® CHO Reagent  consistently outperformed Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagents. 

DNA-In® CHO Transfection Reagent  Quick-Start Protocol

DNA-In CHO protocol


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