DNA-In® Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. These cell-specific reagents offer maximum DNA delivery in targeted common cell lines. Features
Optimized for maximum transfection efficiency and cell viability in A549 cells.
One-step process: simply add DNA-In® A549 Transfection Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells
Minimal toxicity - no need to replace medium after transfection
Maximum Transfection Efficiency in A549
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Above, A549 cells were plated at 70% confluency and were transfected with the EF-1 alpha promoter-GFP 24 hours after plating. DNA-In® A549 transfected cells (left image) show significantly higher expression vs LF3K transfected cells (right image).
During its development and validation process, DNA-In® A549 Transfection Reagent underwent extensive testing in many primary and cultured cells. In a head-to-head comparison study, DNA-In A549 Reagent consistently outperformed Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagents.
The following charts show side-by-side comparisons of DNA-In® A549, Lipofectamine® 2000 (LF2K) and Lipofectamine® 3000 (LFK) in A549 cells:
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