DNA-In® is the newest generation transfection product developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. DNA-In® is a novel cationic transfection reagent which balances high transfection and expression levels with low toxicity. DNA-In® works on a very broad range of adherent cells including most passaged cell lines and many primary cells.
Product sheet
Features
Fast, simple, easy-to-follow protocol
One-step process: simply add DNA-In® Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells
Minimal toxicity - no need to replace medium after transfection
During its development and validation process, DNA-In® Reagent underwent extensive testing in many primary and cultured cells. In a head-to-head comparison study, DNA-In® consistently outperformed the Lipofectamine® 2000 transfection reagent.
Cells were transfected with a plasmid, containing EmeraldGFP behind an EF1α promoter, with either DNA-In® or Lipofectamine®2000, at several different lipid:DNA ratios and examined ~44 hours post-transfection. Representative images of the best conditions for each transfection reagent are shown.
DNA-In® has a significantly lower cell toxicity compared to traditional transfection reagents such as Lipofectamine® and Lipofectamine® 2000. In the many cell lines tested, DNA-In® shows a broad range of effective dosage while maintaining minimal cell toxicity. This low cell toxicity allows a larger dosage and time window for optimized transfection results.
The following charts show side-by-side comparisons of DNA-In® and Lipofectamine® 2000 (LF2K) in commonly used cell lines and primary cells:
In 293 cells, DNA-In® maintains high cell viability throughout the tested dosage range, while LF2K has a significant cell toxicity issue when used in dosages over 3µl.
In A549 cells, DNA-In® has a significantly higher transfection efficiency and a lower cell toxicity while LF2K performs poorly in both parameters.
In human fibroblast cells, DNA-In® has a significantly higher transfection efficiency and a lower cell toxicity while LF2K performs poorly in both parameters.
In HeLa cells, DNA-In has a significantly higher transfection efficiency and a lower cell toxicity while LF2K performs poorly in both parameters.
In HepG2 cells, DNA-In has a significantly higher transfection efficiency and a lower cell toxicity while LF2K performs poorly in both parameters.
In MCF7 cells, DNA-In has a significantly higher transfection efficiency and a lower cell toxicity while LF2K transfection efficiency is lower and is toxic when used in dosages higher than 2µl.
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