外泌体（Exosome）发现于1986年，是一种直径约30~100nm的双层膜囊泡状结构小体，可由机体内多种细胞如免疫细胞、干细胞、心血管细胞、网织红细胞、血小板、神经细胞和肿瘤细胞等主动分泌产生，exosomes广泛分布于外周血、尿液、唾液、乳汁、腹水、羊水等体液中。

在外泌体研究中，外泌体的分离提取是一直困扰广大研究人员的一大难题。外泌体领域资深科学家Clotilde Théry所建立的超速离心提取法一直被奉为外泌体提取的“金标准”。但超速离心法由于需要样本量大，耗时长，依赖特定仪器，提取量低，获得外泌体纯度低等缺点，大大降低了研究人员的研究效率。随着外泌体研究的不断发展，一些商品化试剂盒应运而生，这些试剂盒大多采用PEG沉淀原理，只需在半个小时左右的时间就可完成提取。但由于提取过程中PEG的加入，使获得的外泌体中含有PEG偶联物，严重影响后续实验的进行。另外，无论是超速离心法还是PEG沉淀试剂盒，所分离的外泌体中都含有大量的白蛋白，这也给后续的实验造成很大的干扰。

产品列表

Application

Col-Tgel is a tailorable 3D cell culture matrix for tumor cell, stem cell and primary cell. We provide soft, medium and stiff gel for your different cell types.

It provides collagen backbone for cell attachment and allows customizing matrix components by additional extracellular matrix, growth factors, cytokines, and/or hormones for optimizing three dimensional cell culture.

This product is for research use only.

Col-Tgel是一种可定制的肿瘤细胞、干细胞和原代细胞三维细胞培养基质。我们为您的不同细胞类型提供软、中、硬凝胶。

它为细胞附着提供胶原骨架，并允许通过额外的细胞外基质、生长因子、细胞因子和/或激素定制基质成分，以优化三维细胞培养。

本产品仅供研究使用。

Stiffness selection for different cell types

Formation of tumor spheroids in Col-Tgel

Fang, JY., etc. PloS one 9, no. 8 (2014): e105616.

For immunohistochemistry and immunocytochemistry method, please refer to Page 2 of this publication.

Unique Features

 Handle at room temperature  (no need on ice)

·Save money:  30% below market price

·Save time: No hanging drop, rotating wall vessel bioreactors

·3 stiffness(unique feature): to fit most cell types

·2 mL aliquotes: avoid freeze-thaw cycle - user friendly

· Cytokine / Hormon free: no background

· Flexible:

-Col-Tgel can be customized for different experiments.
- Geometry:  3D embedding, 2D seeding on the top of hydrogel, or unique molds.

-   Sample size and quantity:  6- to 96- well with/without tissue culture insert or transwell.
-   Matrix component and stiffness:  collagen-based with different yield strength (i.e. Soft, Med and Stiff).
-   Transparency:  easy to observe directly under microscopes and can apply with immunohistochemistry directly.

Col-Tgel is a tailorable collagen-based, cell remodelable hydrogel system. It not only can be used a carrier for cell or drug delivery, but also in vitro cell culture platforms. Besides, it contains cell essential nutrients, and provides a stable and durable platform for long-term studies such as cell-cell, cell-matrix interactions, and etc.

Shipping / Storage

Ship at room temperature. Store Component A (gel) at 4oC, and store Component B (crosslinker) at -80oC for 9 months shelf life, or at -20oC for 3 months, or at 4oC for 1 month. Avoid freeze-thaw cycle for Component B.

Shelf Life

Component A: 12 months at 4oC

Component B: 9 months at -80oC; 3 months at -20oC; 1 month at 4oC

Citations

Citation 1:

Kuwahara, Kenrick, Zhi Yang, Ginger C. Slack, Marcel E. Nimni and Bo Han. Cell delivery using an injectable and adhesive transglutaminase–gelatin gel. Tissue Engineering Part C: Methods 16, no. 4 (2009): 609-618.

Citation 2:

Kuwahara, Kenrick, Josephine Y. Fang, Zhi Yang and Bo Han. Enzymatic crosslinking and degradation of gelatin as a switch for bone morphogenetic protein-2 activity. Tissue Engineering Part A 17, no. 23-24 (2011): 2955-2964.

Citation 3:

Fang, Josephine, Zhi Yang, ShihJye Tan, Charisse Tayag, Marcel E. Nimni, Mark Urata and Bo Han. Injectable gel graft for bone defect repair. Regenerative medicine 9, (2014): 41-51.

Citation 4:

Tan, ShihJye, Josephine Y. Fang, Zhi Yang, Marcel E. Nimni and Bo Han. The synergetic effect of hydrogel stiffness and growth factor on osteogenic differentiation. Biomaterials 35, no. 20 (2014): 5294-5306.

Citation 5:

Fang, Josephine Y., Shih-Jye Tan, Zhi Yang, Charisse Tayag and Bo Han. Tumor Bioengineering Using a Transglutaminase Crosslinked Hydrogel. PloS one 9, no. 8 (2014): e105616.

Citation 6:

Shih-Jye Tan, Fang, Josephine Y., Y. Wu, Zhi Yang, G. Liang and Bo Han. Muscle tissue engineering and regeneration through epigenetic reprogramming and scaffold manipulation. Sci Rep. 2015 Nov 9;5:16333. doi: 10.1038/srep16333.

Citation 7:

Lee G, Kim HJ, Kim HM. RhoA-JNK Regulates the E-Cadherin Junctions of Human Gingival Epithelial Cells. J Dent Res. 2015 Dec 3. pii: 0022034515619375. [Epub ahead of print]

Citation 8:

Fang JY, Tan SJ, Wu YC, Yang Z, Hoang BX, Han B. From competency to dormancy: a 3D model to study cancer cells and drug responsiveness. J Transl Med. 2016 Feb 4;14(1):38. doi: 10.1186/s12967-016-0798-8.

* Different names (TGase gel, TG gel) were used in old publications. They referred to the same 3D cell culture gel, and we finally name it as Col-Tgel.

See all 101Bio's citation papers, click here

默瑞生物

默瑞(上海)生物科技有限公司，作为专业的生命科学产品推广与服务商，以“创新，专业，共赢”的理念，不断强化服务体系，提升客户体验，结合国内外优质产品提供高标准完整解决方案，帮助科研客户和生物技术企业高效创新，加速转化，创造更多优质产品，提升社会福祉，实现多方共赢。

默瑞生物创立以来,已经陆续导入TwistDx,enzymatics,KAPA BIOSYSTEMS,BioDynami,Jackson,Echelon,ChromoTek,Nanocs,Biotechrabbit,Lee Biosolutions, magtivio,美的医疗冰箱,FOUR E'S科学仪器等优质国际品牌，逐步培育成为专业用户的选择。

代理试剂产品有：TwistDx恒温扩增试剂盒,Echelon脂类研究试剂,KAPA BIOSYSTEMS二代测序试剂, Enzymatics二代测序酶,Jackson ImmunoResearch抗体,ChromoTek荧光蛋白及抗体,Horizon Discovery测序标准品,Biomatik公司Elisa试剂盒,Nanocs PEG修饰剂,Spectrumlabs透析袋,Polysciences微球,JenaBioscience分子试剂,101BioLifespan Technologies硫酸葡聚糖ELISA试剂盒及抗体,Lee Biosolutions抗体,Emfret心血管研究抗体,Magtivio B.V.DNA提取磁珠,Biontex Laboratories转染试剂,Canvax™细胞凋亡检测试剂盒,Matriks Biotek®生物药物检测试剂盒,Immunostep流式细胞术抗体，Fuller Laboratories感染性疾病检测试剂盒,SICGEN抗体,biotechrabbit酶及抗体等。