TwistDx,echelon,Jackson,enzymatics,101BIO,KAPA,ChromoTek,Biotechrabbit,默瑞生物

Echelon最新产品： EV Purification Kit and ExoTracker kits 外泌体纯化和标记试剂盒

作者：Morey　发布时间：2020-11-19　点击次数：2837

ExoClean and ExoTracker

K-4300a, K-4300b, K-4300c, K-4300d (20 tests)

Support: echelon@echelon-inc.com

Description: Simple, quick protocols for labeling (ExoTracker) and isolating (ExoClean) extracellular vesicles (EVs) from biological samples*

Echelon’s ExoClean – EV Purification Kit and ExoTracker kits为标记和纯化外显体和细胞外囊泡（EV）提供了一个简单可靠的系统。（1）The ExoClean – EV Purification Kit (货号：K-4300a )通过将沉淀试剂与微离心柱配对，取代了与基于超离心的纯化相关的时间和设备成本，此外可以去除与其他沉淀技术相关的有害污染物。https://echelon-inc.com/product/exoclean-ev-purification-kit/?mc_cid=a56b7d5013&mc_eid=c05a3cf4fc

（2）The ExoTracker kits (货号：K-4300b -d) 使该系统的实用性更进一步，可以用膜或RNA特异性标签标记EV。

产品列表

Catalog #

Description

Quantity

ExoClean (K-4300a)

K-4301	EV Precipitation Reagent	30 mL
K-SPIN	Microcentrifuge Columns	20 columns

ExoTracker - Membrane (K-4300b)

K-4300a	ExoClean	1 kit
K-4302	EV Membrane Label	150 µg

ExoTracker - RNA (K-4300c)

K-4300a	ExoClean	1 kit
K-4303	EV RNA Label	20 µL

ExoTracker - Total (K-4300d)

K-4300a	ExoClean	1 kit
K-4302	EV Membrane Label	150 µg
K-4303	EV RNA Label	20 µL

操作流程：

1：Collect EV sample

2：Centrifuge at 2000g for 10 min to remove any debris or cells.

3：Transfer supernatant and precipitate cleared sample with-K4301 for 30 minutes at room temperature with mixing

4：Centrifuge precipitated sample at 1500g for 30 minutes at room temperature.

5：Discard the supernatant fraction and resuspend the precipitated pellet in 1x PBS.

6：Apply the resuspended pellet to the equilibrated K-SPINcolumn and incubate for 5 minutes at room temperature.

7：Centrifuge the column at 500g for 30 seconds at room temperature.

8：Collect and examine the eluted, purified EVs.

注：Exosomes and extracellular vesicles (EVs)-胞外体和胞外小泡

发表文献：

1. Konoshenko MY, Lekchnov EA, Vlassov AV, Laktionov PP. Isolation of Extracellular Vesicles: General Methodologies and Latest Trends. Biomed Res Int. 2018;2018:8545347.

2. Li P, Kaslan M, Lee SH, Yao J, Gao Z. Progress in Exosome Isolation Techniques. Theranostics. 2017;7(3):789-804.

3. Deregibus MC, Figliolini F, D’Antico S, Manzini PM, Pasquino C, De Lena M, et al. Charge-based precipitation of extracellular vesicles. Int J Mol Med. 2016;38(5):1359-66.

默瑞生物：

默瑞(上海)生物科技有限公司，作为专业的生命科学产品推广与服务商，以“创新，专业，共赢”的理念，不断强化服务体系，提升客户体验，以莫纳生物产品为主线，结合国内外优质产品提供高标准完整解决方案，帮助科研客户和生物技术企业高效创新，加速转化，创造更多优质产品，提升社会福祉，实现多方共赢。

默瑞生物创立以来,已经陆续导入TwistDx,博日

Jackson,izon,Biontex,Immunostep,101Bio,enzymatics,AATBioquest,KAPABIOSYSTEMS,BioDynami,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等优质国际品牌，逐步培育成为专业用户的首选

扫码关注我们

默瑞4006-400-850

售前 : info@moreybio.com

售后：tech@moreybio.com

官网：www.moreybio.com