DiscoverX 检测试剂
作者：Morey 发布时间：2020-03-24 点击次数：2134
DiscoverX 检测试剂
DiscoverX提供同时具有化学发光和荧光读数的检测试剂，以满足您的药物发现计划的不同需求。DiscoverX的化学发光检测是基于专有的酶片段互补（EFC）技术，而荧光检测是基于激活的荧光染料。这些检测试剂被优化用于多种应用，例如检测基于细胞的蛋白质-蛋白质相互作用、复合蛋白质结合、易位和第二信使分析。DiscoverX的检测试剂是为DiscoverX细胞系设计的，包括cAMP Hunter
，InCELL 或PathHunter细胞系、InCELL分析和ProLabel/ProLink （PL/PK）全细胞内的表达或克隆载体。
，InCELL 或PathHunter细胞系、InCELL分析和ProLabel/ProLink （PL/PK）全细胞内的表达或克隆载体。| Product | Cat No. | Compatible Cell Lines |
|---|---|---|
| PathHunter Detection Kit | 93-0001 Series | PathHunter β-Arrestin |
| PathHunter Internalization | ||
| PathHunter Pharmacotrafficking | ||
| PathHunter Receptor Tyrosine Kinase | ||
| PathHunter Cytosolic Tyrosine Kinase | ||
| PathHunter Pathway | ||
| PathHunter Nuclear Hormone | ||
| PathHunter Parentals | ||
| PathHunter Flash Detection Kit | 93-0247 Series | PathHunter Dimerization |
| PathHunter Pharmacotrafficking | ||
| HitHunter® cAMP Assay for Biologics | 90-0075LM Series | cAMP Hunter Gs-/Gi-Coupled Cell Line |
| HitHunter cAMP Assay for Small Molecules | 90-0075SM Series | |
| KILR® Detection Kit | 97-0001 Series | KILR Target Cell Lines/Pools |
| PathHunter Bioassay Detection Kit | 93-0933 Series | PathHunter Bioassay |
| PathHunter Bioassay ED Detection Kit | 93-1043 Series | |
| InCELL Detection Kit | 96-0079 Series | InCELL Hunter Cell Lines |
| Product Change Notice | ||
| HitHunter cGMP Assay | 90-0039 Series |
Detection Kits for Functional Confirmation of EFC Component Expression
| Product | Cat No. | Intended Use |
|---|---|---|
| EFC BlocDetect™ Kit | 92-0004 | Detect false positive results by identifying compounds that inhibit β-galactosidase complementation or β-galactosidase activity |
| ProLink Detection Kit | 92-0006 Series | Detect ProLink-tagged protein in parental cells expressing ProLink Vectors |
| PathHunter ProLabel Detection Kit | 93-0180 Series | Detect ProLabel-tagged protein in parental cells expressing ProLabel Vectors |
| PathHunter EA Detection Kit | 93-0183 Series | Detect large protein fragment of β-galactosidase (Enzyme Acceptor, EA) in parental cells expressing EA such as ENDO-EA, MEM-EA, NUC-EA, β-arrestin EA, and SH2-EA |
| PathHunter PL/PK Detection Kit | 93-0812 Series | For functional confirmation of EFC components with PathHunter Pathway cell lines |
Fluorescence Detection Products
| Product | Cat No. | Intended Use |
|---|---|---|
| HitHunter Calcium No WashPLUSAssay | 90-0091 Series | Monitor GPCR and ion channel activation using Native Calcium Gq-Coupled Cell Lines |
| Coelenterazine h | 90-0084 | |
| ADP Hunter™ Plus | 90-0083 Series | Detect kinase activity by monitoring ATP depletion |
| ADP Quest™ | 90-0071 Series | Kinase and inhibitor characterization for kinetic or endpoint analysis |
